6th TRAINING SCHOOL - Expression, purification and basic characterization of target protein samples for drug binding studies

Description

Description. The successful large-scale production and purification of a target protein allows to initiate all the
biochemical and biophysical studies that can lead to the characterization of the potency and binding mode of
potential inhibitors, substrates or ligands. This training school aims to provide basic knowledge and hands-on
experience on protein expression protocols using bacterial cells, protein purification methods, and biophysical
techniques for the rapid assessment of protein stability and purity. The course will feature a combination of
lectures and practical laboratory activities.
The participants will familiarize themselves with the most common procedures for large scale protein
expression in bacteria, standard protein purification protocols using liquid chromatography techniques (Affinity,
Size-Exclusion, and Ion-Exchange Chromatography) as well as some basic biophysical techniques for the
immediate, rapid assessment of the quality of the protein thus produced. These include: Polyacrylamide Gel
Electrophoresis (SDS PAGE, both under native and denaturing conditions), Circular Dichroism (CD), Differential
Scanning Fluorimetry (DSF), and Mass Spectrometry (MS).

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You are invited to submit your filled Application Form (download it here) together with your short CV (no longer than one page) and an endorsement letter from the supervisor on institutional head paper by the 10/07/2025 at the following address: Atis Jekabsons (atis@osi.lv) and Sheraz Gul (Sheraz.Gul@itmp.fraunhofer.de). 

Successful applicants will need to create an e-COST account ( https://e-services.cost.eu) and will receive an official invitation.